Measuring femoral condyle length

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Measuring femoral condyle length

modz9636
Hello,

I am looking for a way to measure the cartilage-covered area of a mouse
femur.  I have sagittal sections of the mouse joints stained with H&E.

Ultimately, I want to measure the joint surface and then calculate the
middle 50%. So I would have Anterior 25%, Middle 50%, and Posterior 25%.  I
used the Bezier curve to draw the adjusted arc, but I don't know how to
calculate the length and then figure out the percentages.

If you have any suggestions, it would be much appreciated!

Thank you so very much!

Sincerely,
Merissa