Hi,
you may give our ridge detection plugin a try:
http://fiji.sc/Ridge_DetectionCheers,
Thorsten
Am Montag, den 06.07.2015, 11:18 -0700 schrieb clach:
> Hi, I'm looking to measure the length of curved lumens in c. elegans.
>
>
> I have a tiff stack of around 30 images, and while it is
> possible/easy to
> measure the length in 2 dimensions for one image in the stack, I want
> to be
> able to account for the length that the lumen curves in the Z
> dimension. I
> would also like this process to be relatively automatic so I can use
> it for
> all of my files.
>
> I have been attempting to use binary/skeletonize/analyze skeleton,
> but it
> seems the lumen is too thick (?) resulting in skeletons that do not
> accurately represent the lumens (instead of giving one line for the
> lumen,
> it sometimes gives two for the perimeter or border of the lumen).
> Additionally, I am not sure of the units in which "length" is given
> when I
> use analyze skeleton... and is that the entire length of the skeleton
> found
> in all images in the stack?
>
> If skeletonize is not the best method, please let me know! Thanks for
> any
> and all help!
>
>
>
> --
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>
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