Measuring tubular length, branching points and branch length

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Measuring tubular length, branching points and branch length

Pbl
Hello everyone,

for the analysis of branching morphogenesis I am trying to measure the length of tubular structures as well as the angle and length of branches. The raw data is a tiff image which i converted into a binary. I tryed to sceletonise and measure the length of each line but i failed. Can someone tell me how i can measure the individual lines? Another Problem may be that the tubules are not straight lines but curves.

Best