Merging brightfield and RGB images

[Nikhil Angelo]

Hello everyone,

I am trying to merge a brightfield images with images I have RGB-merged.  If
I try to put in the merged image with the brightfield image in the
red/green/blue channel, the brightfield image is then converted to whichever
channel into which it was loaded.

I attempted to find a plugin that would enable me to merge brightfield
images with another image, but to no avail.  Does anyone have such a
plugin?  If so, where could I find it?

Thank you!
Nikhil Angelo

--
Nikhil Angelo
6985 Snow Way Dr.
Campus Box 6933
St. Louis, MO 63130
[hidden email]

[Albert Cardona]

Hi Nikhil,

In my experience, it's much better to first convert the brightfield
image to RGB, and paste the colored fluorescent channels into its RGB
channels. The other way around, like you are trying to do, will render
images with strange colored backgrounds.

Here is how I'd do it:


1) convert your brighfield image to an RGB Image (Image -> Type -> RGB
Color)
    and then to an RGB stack (Image -> Type - > RGB Stack)
2) convert your color image to RGB Stack (Image -> Type - > RGB Stack)
3) open the Paste Control (Edit -> Paste Control...)
4) select "Max"
5) Copy the color channel you want to transfer from the color image
(which now is a stack of 3 slices)
6) paste into the desired channel of the RGB Stack of the brighfield image.
7) convert the RGB Stack to an RGB (Image -> Type -> RGB Color)


The Max operation chooses the largest value of both pixels, so that any
two images can be merged very nicely. You can of course experiment with
other paste ptions, and with preadjusting the intensity of the image
being pasted.


Albert

[Arne Seitz-2]

Hi,

the plugin you are looking for can be found there.

http://rsb.info.nih.gov/ij/plugins/rgb-gray-merge.html

Cheers Arne


_____________________________________
Arne Seitz
Advanced Light Microscopy Facility
EMBL Heidelberg

[Rainer Kohler-2]

Try this one

http://rsb.info.nih.gov/ij/plugins/rgb-gray-merge.html

RGB Gray Merge

     Authors: Eugene Tkachenko ([hidden email])
     and Justin D. Pearlman
     Date: 2001/12/12
     Source: RGB_Gray_Merge.java
     Installation: Download RGB_Gray_Merge to the plugins folder
and restart ImageJ.
     Description: Combines up to four 8-bit grayscale images or
stacks (gray, red, green and blue) into one RGB image or stack.

>Hello everyone,
>
>I am trying to merge a brightfield images with images I have RGB-merged.  If
>I try to put in the merged image with the brightfield image in the
>red/green/blue channel, the brightfield image is then converted to whichever
>channel into which it was loaded.
>
>I attempted to find a plugin that would enable me to merge brightfield
>images with another image, but to no avail.  Does anyone have such a
>plugin?  If so, where could I find it?
>
>Thank you!
>Nikhil Angelo
>
>--
>Nikhil Angelo
>6985 Snow Way Dr.
>Campus Box 6933
>St. Louis, MO 63130
>[hidden email]

--
Rainer Kohler, Ph.D
Microscopist
Center for Molecular Imaging Research
Massachusetts General Hospital/ Harvard Medical School
Building 149, 13th  Street, Room 5406
Charlestown, MA 02129-2060
Tel. [phone]
[hidden email]




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