Montage with composite pb

Previous Topic Next Topic
 
classic Classic list List threaded Threaded
2 messages Options
Reply | Threaded
Open this post in threaded view
|

Montage with composite pb

JPCLAMME
Hi,

I have a 4 channels x 8 z planes  hyperstack that I visualize as a
composite image.
I tried to do a montage of one of the z plane and what I get is an image
showing  channel 2 to 4 in there right color but channel 1 is replaced by
the composite overlay image.
Also I noticed that when I define the start and end slice for the montage,
it doesn't reefer to the actual slice in the whole hyperstack but to the
channel numbers of the z plane I'm visualizing. Therefore if I define the
end to 6 for example 1 will see the 4 channels + 2 blank images.

Is this normal ?

Thanks,

JP
Reply | Threaded
Open this post in threaded view
|

Re: Montage with composite pb

Wayne Rasband
On May 8, 2009, at 3:08 PM, Jean Pierre CLAMME wrote:

> Hi,
>
> I have a 4 channels x 8 z planes  hyperstack that I visualize as a
> composite image.
> I tried to do a montage of one of the z plane and what I get is an
> image showing  channel 2 to 4 in there right color but channel 1 is
> replaced by  the composite overlay image.
> Also I noticed that when I define the start and end slice for the
> montage,
> it doesn't reefer to the actual slice in the whole hyperstack but to
> the
> channel numbers of the z plane I'm visualizing. Therefore if I define
> the
> end to 6 for example 1 will see the 4 channels + 2 blank images.
>
> Is this normal ?

To create a montage of a single Z plane, use Image>Hyperstacks>Reduce
Dimensionality to extract the Z plane and then run Make Montage. To
create montage of selected Z planes, use Image>Type>RGB Color to
convert the hyperstack to RGB and then run Make Montage.  The Make
Montage command in v1.42o, when it detects a hyperstack, will display
instructions on how to prepare it. The Make Montage command in v1.42o
also acquires a keyboard shortcut: shift+m.

-wayne