Hi,
ImageJ 2.0 (
http://imagejdev.org/), due out in October, will feature an
N-dimensional data engine. However, to work with multi-frequency data, more
is required:
First you need a file reader that can import the data successfully. Maybe
Bio-Formats can (
http://loci.wisc.edu/bio-formats), depending on your file
format.
After that you need an analysis algorithm to process your multi-frequency
data. It is unlikely that you can do much naive image processing on FLIM
data, and I do not know of any ImageJ plugins for multi-frequency data
specifically, but you could write one. Unfortunately I cannot really comment
further because we have not worked with that sort of FLIM data in my group
(we do TCSPC).
Regards,
Curtis
On Tue, Jun 7, 2011 at 4:12 PM, Grad Student <
[hidden email]>wrote:
> Yes.
> Adding more than the current existing dimensions that is X-Y-Z-Time-Color.
>
>
>
> --- On Tue, 6/7/11, Damon Poburko <
[hidden email]> wrote:
>
> > From: Damon Poburko <
[hidden email]>
> > Subject: Re: Multi-Frequency Analysis in FIJI
> > To:
[hidden email]
> > Date: Tuesday, June 7, 2011, 1:53 PM
> > On 6/7/2011 10:41 AM, Grad Student
> > wrote:
> > > Hi All,
> > >
> > > I am interested in using FIJI for multi-frequency data
> > analysis.
> > > Currently, there are only 5 dimensions allowed in
> > FIJI.
> > > Can anyone guide me as to what changes can be made or
> > how I can incorporate more dimensions for multi-frequency
> > analysis?
> > >
> > > Thank you.
> > >
> > Assuming I'm not the only one out there that might not know
> > what you're
> > talking about.... what exactly do you mean by
> > multi-frequency analysis.
> > You have more than X-Y-Z-Time-Color that you want to
> > record/analyze?
> >
> > Damon
> >
> > --
> > Post Doctoral Fellow
> > Tsien Lab, Molecular& Cellular Physiology
> > Stanford University
> >
>
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