Multithreshold question

[Hanna_SN]

Dear Image J users,

I'm new to image j and for last couple of days I'm struggling with a problem
to with I can't find a solution. I would like to group pixels on my image to
few intensity-based classes (based on the histogram) and then assign to each
of the classes different color in the LUT. As a result I wont to get
something like a thermal-map (but not gradient map) showing significant
differences between fluorescence intensity. Unfortunately I can't find a
solution how revalue pixels on a picture so that I get 5-6 classes.

I will be very grateful for any suggestions,
Best Regards,
Hanna

--
Department of Plant Anatomy and Cytology
Faculty of Biology and Environmental Protection
University of Silesia
Jagiellonska Str. 28
40-032 Katowice
Poland

[Mario Faretta]

  Il 9/20/2010 2:14 PM, Hanna Sas Nowosielska ha scritto:

> Dear Image J users,
>
> I'm new to image j and for last couple of days I'm struggling with a problem
> to with I can't find a solution. I would like to group pixels on my image to
> few intensity-based classes (based on the histogram) and then assign to each
> of the classes different color in the LUT. As a result I wont to get
> something like a thermal-map (but not gradient map) showing significant
> differences between fluorescence intensity. Unfortunately I can't find a
> solution how revalue pixels on a picture so that I get 5-6 classes.
>
> I will be very grateful for any suggestions,
> Best Regards,
> Hanna
>

Maybe the k-means clustering plugin
(http://ij-plugins.sourceforge.net/plugins/clustering/index.html) or the
multi-otsu threshold
(http://rsb.info.nih.gov/ij/plugins/multi-otsu-threshold.html) can help you
Mario

--
---PLEASE Note the change in telephone number---

--
Mario Faretta
Department of Experimental Oncology
European Institute of Oncology
c/o IFOM-IEO Campus for Oncogenomics
via Adamello 16 20139 Milan Italy
Phone: ++39-0294375027
email: [hidden email]
http://www.ifom-ieo-campus.it

[Hanna_SN]

Dear Mario,

Thank you for your advice. I think that multi-otsu threshold would be helpful but unfortunately I can't compile it. Whenever I tried to do this (I'm using MBFimage J) the message "cant locate javac" appears.
I tried to install new java and new image j but nothing helps. What am I doing wrong?

Hanna