Wang,
the *.mdb file just keeps track of what lsm files are
in the same folder and lets you have the gallery view
from inside the Zeiss software. All information about
images are in the *.lsm files
You can use the ImageJ [Image/Stacks/Polt Z-axis
Profile] to measure a ROI throughout an image stack.
It wil output the sort of graph your looking for.
Leo.
> > Dear Colleague,
> >
> > I have some stack images to process and these
> images are acquired on
> > Zeiss LSM 510. However, I have two questions yet
> for consultation:
> > 1) How to open the "*.MDB" file using ImageJ.
> These files contain some
> > information of the raw images.
> > 2) In processing the stack images, I can get the
> intensity values over
> > sequential images in the stack upon ROI region.
> However, I don't know
> > how to plot the 2D graph of the sequential
> intensity values (X: image
> > label in the stack; Y: intensity values in ROI
> region of relavant
> > image). ¡¡¡¡¡¡
> >
> > Best wishes,
> > Wang XL
> > 2006-03-24
> >
>
------------------------------------------------------
> > Xu-Long,Wang (PhD Student)
> > School of Life Science and Technology
> > Shanghai Jiao Tong University
> > E-mail:
[hidden email]
> >
>
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