Hi
Many years ago I wrote a simple macro for the old NIH Image. I have never updated it to apply to ImageJ but would like to do so now. Unfortunately, after reviewing the documentation available, I don't see how to do this. I have no background in Java at all. The macro I wrote operates on a series of already opened images, applies a filter to each, applies a math operation between the filtered file and a pre-existing grid, analyzes the resultant image, then does the same with a second grid, analyzes this image too, then closes the image. I can easily do the image analysis on a single image but don't see how to automate the process (e.g. open up one image, analyze it, close it, open the next, etc). I realize that this must have something to do with the batch function, but don't see how to do it. I have included my old macro below. If anyone can help, I would appreciate it. Robert Homer procedure processimage; var g,intval:integer; begin IntVal:=GetNumber('Set Threshold(1--255):',50); for g:=1 to nPics do begin choosePic(g); SetThreshold(IntVal); MakeBinary;Filter('Median');filter('median'); Invert; end; end; procedure chordlength; var g,d,e:integer begin for g:=1 to nPics do begin choosepic(g); d:=picnumber; Open('power hd:applications:graphics:NIH Image 1-60:macros:gridxx'); e:=PicNumber; ImageMath('and',d,e,1,0,'result'); AnalyzeParticles('ignore');Dispose; choosePic(e);Dispose; Open('power hd:applications:graphics:NIH Image 1-60:macros:gridyy'); e:=PicNumber; imageMath('and',d,e,1,0,'Result'); AnalyzeParticles('ignore');Dispose; ChoosePic(e); Dispose; end; end; macro 'Chord excluding edges-pic [A]'; var IntVal,g,d,e,i:integer; sum:real; file:string; begin SetPrecision(2,8); SetParticleSize(15,500000); SetOptions('Area'); ResetCounter; SetScale(1,'pixel',1); processimage; chordlength; SetUser1Label('mean'); file:=WindowTitle; sum:=0; for i:=1 to rCount do sum:=sum+rArea[i]; rUser1[rCount]:=sum/(rCount*1.5); UpdateResults; SetExport('measurements'); Export('chord length', file); end; end; |
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