Dear friends and colleagues,
We just released a new Fiji plugin dedicated to automated & manual spot tracking, TrackMate.
http://fiji.sc/wiki/index.php/TrackMateIt can deal with 2D & 3D data, and offers a few techniques for automated spot segmentation and particle-linking. It can deal with track splitting (''e.g.'' when cells divide) or track merging. It also offers facilities for manual editing spots and tracks, exporting and inspecting results.
Also for developers, TrackMate was developed having in mind extensibility and flexibility. It is made so that if you wish to implement a new tracking or segmentation algorithm, you can re-use TrackMate components like the GUI, avoiding the burden of making one from scratch.
A step-by-step tutorial can get you started and show what you can do with the plugin. The plugin page also contains links to the rest of the documentation.
http://fiji.sc/wiki/index.php/Getting_started_with_TrackMateHope you like it!
Jean-Yves, Nick, Johannes, Ricardo & Spencer
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Jean-Yves Tinevez
PFID - Imagopole
Institut Pasteur
25-28, rue du Docteur Roux
75724 Paris cedex 15
France
tel: +33 1 40 61 35 40