Non-integer RGB values / Color deconvolution

[Rodrigo Gonçalves]

Hi all,


I'm using Color Deconvolution with FIJI and there's something I don't fully understand.


The red, green and blue colors of a RGB image use 8 bits each, which have integer values from 0 to 255, right? Therefore, what is the meaning ofhaving values with 6 digits after the decimal point?

For example when I use Color Deconvolution with “From ROI” option, I get the Vector Matrix with values of e.g.


Red: 9.290228
Green: 8.5856
Blue: 11.380588

Are all those non-integer digits the result of average-like operations over a number of pixels with different, integer RGB values?

Best,
Rodrigo

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[Gabriel Landini]

On Wednesday 15 Apr 2015 18:18:35 Rodrigo Gonçalves wrote:

> For example when I use Color Deconvolution with “From ROI” option, I get the
> Vector Matrix with values of e.g.
>
>
> Red: 9.290228
> Green: 8.5856
> Blue: 11.380588
>
> Are all those non-integer digits the result of average-like operations over
> a number of pixels with different, integer RGB values?

Yes. Since you are using an ROI to define one pure colour, it is likely to
have more than one pixel. The average of integers is not necessarily another
integer.
I hope it clarifies it.

Gabriel

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