Normalize White Balance Histology

[redcarnations]

Hi all,

i imaged HE-stained sections two separate days. the white balance on the 2 images is off, the first day images have more of a bluish-white hue and the second day images have more of a yellowish-white hue. i would like to normalize all images from both days and make them have the same hue along with the same red/blue hemotoxylin and eosin stained colors. how to do this on imagej?
i tried Stack Normalize plugin but the white color is not the same across all 3 images.
help is much appreciated.

[Gabriel Landini]

On Thursday 13 Mar 2014 12:01:01 you wrote:
> i imaged HE-stained sections two separate days. the white balance on the 2
> images is off, the first day images have more of a bluish-white hue and the
> second day images have more of a yellowish-white hue. i would like to
> normalize all images from both days and make them have the same hue along
> with the same red/blue hemotoxylin and eosin stained colors. how to do this
> on imagej?
> i tried Stack Normalize plugin but the white color is not the same across
> all 3 images. help is much appreciated.

Have a look here:
http://imagejdocu.tudor.lu/doku.php?id=howto:working:how_to_correct_background_illumination_in_brightfield_microscopy

Cheers

Gabriel

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[redcarnations]

thank you, that worked!