Nucleus counter

[Hongmei Yu]

Hi everyone,
 I am looking for a plugins called "Nucleus counter". but I can never find it.
Could someone tell me whether it exists or where I could find it?  I want to
try it see how much more I could improve the quantification of cells?
 this is what I find about this plugins in some website ----
"The particle analysis can be automated via plugins or macros once the correct
threshold value and particle size range has been determined for your objects of
interest. See the “Plugins/Particle Analysis/ Nucleus counter” plugin and its
source code to customise it to your images."

Thanks.

Hongmei

[Tony Collins-2]

The website is the "Online Manual for the WCIF-ImageJ Collection". It
says this at the top which is also a link to the collection.
Please read the "About this manual" section of this website.

Tony

Hongmei Yu wrote:

>Hi everyone,
> I am looking for a plugins called "Nucleus counter". but I can never find it.
>Could someone tell me whether it exists or where I could find it?  I want to
>try it see how much more I could improve the quantification of cells?
> this is what I find about this plugins in some website ----
>"The particle analysis can be automated via plugins or macros once the correct
>threshold value and particle size range has been determined for your objects of
>interest. See the “Plugins/Particle Analysis/ Nucleus counter” plugin and its
>source code to customise it to your images."
>
>Thanks.
>
>Hongmei
>  
>

--
Tony Collins, Ph.D.
Facility Manager
Wright Cell Imaging Facility
Toronto Western Research Institute
13-407 McLaughlin Pavilion
399 Bathurst Street
Toronto, ON. M5T 2S8
tel. [phone] fax: [phone]
http://www.uhnresearch.ca/wcif

[Martin du Saire]

I have a related question:

Once one ends up with an algorithm for counting blobs (nuclei,
mosquito eggs, etc.)  how should one go about evaluating the efficacy
of the procedure, and are there any major statistical pitfalls to
avoid?  Just looking for a shove in the right direction.

Thanks.

Martin

At 08:47 AM 10/19/2005, you wrote:

>The website is the "Online Manual for the WCIF-ImageJ Collection".
>It says this at the top which is also a link to the collection.
>Please read the "About this manual" section of this website.
>
>Tony
>
>Hongmei Yu wrote:
>
>>Hi everyone,
>>I am looking for a plugins called "Nucleus counter". but I can never find it.
>>Could someone tell me whether it exists or where I could find it?  I want to
>>try it see how much more I could improve the quantification of cells?
>>this is what I find about this plugins in some website ----
>>"The particle analysis can be automated via plugins or macros once
>>the correct
>>threshold value and particle size range has been determined for
>>your objects of
>>interest. See the "Plugins/Particle Analysis/ Nucleus counter" plugin and its
>>source code to customise it to your images."
>>
>>Thanks.
>>
>>Hongmei
>>
>
>--
>Tony Collins, Ph.D.
>Facility Manager
>Wright Cell Imaging Facility
>Toronto Western Research Institute
>13-407 McLaughlin Pavilion
>399 Bathurst Street
>Toronto, ON. M5T 2S8
>tel. [phone] fax: [phone]
>http://www.uhnresearch.ca/wcif

[Michael Cammer]

Every time I write an automated routine I like to test with computer
generated data where I know what the answer is and with at least one
(typically a few, but who has the time?) real biological samples where I've
done the analysis manually or by another method.
-Michael

At 12:29 PM 10/19/05 -0500, you wrote:

>I have a related question:
>
>Once one ends up with an algorithm for counting blobs (nuclei, mosquito
>eggs, etc.)  how should one go about evaluating the efficacy of the
>procedure, and are there any major statistical pitfalls to avoid?  Just
>looking for a shove in the right direction.
>
>Thanks.
>
>Martin

____________________________________________________________________________
Michael Cammer   Analytical Imaging Facility   Albert Einstein Coll. of Med.
Jack & Pearl Resnick Campus      1300 Morris Park Ave.     Bronx, NY  10461
[phone]       Fax:  [phone]      URL:  http://www.aecom.yu.edu/aif/
   **This electronic transmission contains information that is privileged.**

[Martin du Saire]

Only a few?  Isn't it necessary to determine statistically the
reliability of the automated routine?

Martin

At 09:48 AM 10/21/2005, you wrote:

>Every time I write an automated routine I like to test with computer
>generated data where I know what the answer is and with at least one
>(typically a few, but who has the time?) real biological samples
>where I've done the analysis manually or by another method.
>-Michael
>
>At 12:29 PM 10/19/05 -0500, you wrote:
>>I have a related question:
>>
>>Once one ends up with an algorithm for counting blobs (nuclei,
>>mosquito eggs, etc.)  how should one go about evaluating the
>>efficacy of the procedure, and are there any major statistical
>>pitfalls to avoid?  Just looking for a shove in the right direction.
>>
>>Thanks.
>>
>>Martin
>
>____________________________________________________________________________
>Michael Cammer   Analytical Imaging Facility   Albert Einstein Coll. of Med.
>Jack & Pearl Resnick Campus      1300 Morris Park Ave.     Bronx, NY  10461
>[phone]       Fax:  [phone]      URL:  http://www.aecom.yu.edu/aif/
>   **This electronic transmission contains information that is privileged.**