Object based colocalization analysis

[Dipanjana Ghosh]

Dear all,

I would like to do object based co-localization analysis. Came across the JACop plugin which has an option of "Object based methods". It is generating the following information after the analysis.

Label   Centre_A_n° Centre_B_n° d(A-B) reference_dist phi theta XA YA ZA XB YB ZB
Co-localization 48 38 99.649 160.286 2.356 1.571 799.5 683 0 799 682.5 0
Co-localization 54 39 40.323 160.286 1.69 1.571 779.242 704.576 0 779.208 704.292 0

Being a beginner in the field I need some advice on how from these outputs we can calculate Co-localization. Is there any tutorial on this or any write up that can help?

Thanking you

Regards,
Dipanjana

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[Arne Seitz]

The following publications might be a good starting point.

https://www.ncbi.nlm.nih.gov/pubmed/17210054

http://imagejdocu.tudor.lu/_media/plugin/analysis/jacop_2.0/just_another_colocalization_plugin/jacop_ijconf2008.pdf

Best
Arne

-----Original Message-----
From: ImageJ Interest Group <[hidden email]> On Behalf Of Dipanjana Ghosh
Sent: Dienstag, 14. Mai 2019 08:34
To: [hidden email]
Subject: Object based colocalization analysis

Dear all,

I would like to do object based co-localization analysis. Came across the JACop plugin which has an option of "Object based methods". It is generating the following information after the analysis.

Label   Centre_A_n° Centre_B_n° d(A-B) reference_dist phi theta XA YA ZA XB YB ZB
Co-localization 48 38 99.649 160.286 2.356 1.571 799.5 683 0 799 682.5 0
Co-localization 54 39 40.323 160.286 1.69 1.571 779.242 704.576 0 779.208 704.292 0

Being a beginner in the field I need some advice on how from these outputs we can calculate Co-localization. Is there any tutorial on this or any write up that can help?

Thanking you

Regards,
Dipanjana

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html

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ImageJ mailing list: http://imagej.nih.gov/ij/list.html