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This post was updated on .
Dear All,
I'm brand new here and also am relatively new to ImageJ, having worked on it for two weeks. I have been watching the YouTube videos and scouring the web to learn how to use this software to obtain the results I need. What I want to do is measure how width of cilia change along their length, from microscopy images.
One way of measuring width is by taking cross sectional intensity profiles (using the same ROI) along the cilia, then compiling the results. But unless I use a ROI that have a line width of one pixel, I can't get all the possible measurements- and doing it that way is incredibly laborious and impossible with the 50 images or so, I'll generate. The reason I'm using intensity profiles to measure width, is that with the same data, I could work out protein distribution across the cilia at that instantaneous point.
Attached is Image_185, an example of my cilia images. Focus on the picture in the bottom half, containing red and green. I've set the scales and split the colours- into red and green (different proteins) and set a threshold of 20-255.
Is there any way, using the split red and green images, to accurately measure cilia width (along their lengths) using intensity profiles, without having to inaccurately use a ROI one pixel wide and generate measurements hundreds of times and then compile results for a single image. Basically, at the end of this, I want to be able draw the graph, y-axis cilia width, x-axis cilia length (from proximal end).
Very happy to provide more info on this should you need it.
Fingers crossed you can think of a way to solve this issue!!
Best wishes,
ND
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