Opening ND2 files in FIJI

[danibodor]

Hi there,

I have been trying to open *.nd2 files from Nikon Elements in FIJI using the BioFormats Importer. However, I get a range of different errors when doing this, depending on the settings that I try to use.

When trying as 'standard ImageJ' or 'Hyperstack' format:
        java.lang.ArithmeticException: / by zero
        at loci.formats.in.NativeND2Reader.initFile(NativeND2Reader.java:503)
        at loci.formats.FormatReader.setId(FormatReader.java:1058)
        at loci.formats.DelegateReader.setId(DelegateReader.java:216)
        at loci.plugins.in.ImportProcess.initializeFile(ImportProcess.java:475)
        at loci.plugins.in.ImportProcess.execute(ImportProcess.java:138)
        at loci.plugins.in.Importer.showDialogs(Importer.java:125)
        at loci.plugins.in.Importer.run(Importer.java:77)
        at loci.plugins.LociImporter.run(LociImporter.java:77)
        at ij.IJ.runUserPlugIn(IJ.java:185)
        at ij.IJ.runPlugIn(IJ.java:152)
        at ij.IJ.runPlugIn(IJ.java:141)
        at HandleExtraFileTypes.openImage(HandleExtraFileTypes.java:343)
        at HandleExtraFileTypes.run(HandleExtraFileTypes.java:52)
        at ij.IJ.runUserPlugIn(IJ.java:185)
        at ij.IJ.runPlugIn(IJ.java:152)
        at ij.IJ.runPlugIn(IJ.java:141)
        at ij.io.Opener.openWithHandleExtraFileTypes(Opener.java:413)
        at ij.io.Opener.openImage(Opener.java:288)
        at ij.io.Opener.openImage(Opener.java:309)
        at ij.io.Opener.open(Opener.java:138)
        at ij.io.Opener.openAndAddToRecent(Opener.java:224)
        at ij.plugin.DragAndDrop.openFile(DragAndDrop.java:176)
        at ij.plugin.DragAndDrop.run(DragAndDrop.java:152)
        at java.lang.Thread.run(Thread.java:662)


When trying in 'Image 5D' or 'View 5D' format:
        java.lang.ArithmeticException


Is there anyone that has had this problem before and solved it, or knows how to solve this problem?

Thanks!

Dani

[mike]

We have also had mixed problems with opening nd2s.

On macs, LOCI 4.4 works well for some users but not others. When it didn't work, going edit/options/reset seemed to work for most people. I am not sure why.

we have not managed to open nd2 on 64bit windows and normal PCs, even with LOCI 4.4. We think the problem is with JAVA, but have not figured it out yet...

It must be a clue that the same imagej/loci works on some computers but not - around the world, or in my building. I am not good enough at IT to figure out, but I think it is a java problem.

I'd be interested in knowing how to figure this out too,

Mike