Hi EV,
> A student is trying to open some fluorescence images he took using the
> latest version of imageJ (FIJI) and the screen shows up totally black.
Did you see this?
http://imagej.net/Frequently_Asked_Questions#The_image_I_loaded_is_displayed_all_black.21_But_it_is_not_black.21> when opening fluorescence images they look really bright and grainy,
> not at all like they looked on the original monitor when I took the
> image.
Also likely a Brightness/Contrast issue. Shift+C, change the display min
and max.
In general, when analyzing data, it is more quantitative (and hence much
better) to rely on the numbers than on your eyes. Mouse over the image to
"probe" the data—numbers will appear in the ImageJ status bar area. Check
whether these numbers match across the two machines that differ visually.
> I also notice that when I measure fluorescence (Analyze->Measure), the
> mean values that I get are very different from those used in earlier
> imageJ versions.
That is more worrisome. You can use the Help > Update ImageJ command to
switch versions of ImageJ, including downgrading to an older version that
worked for you before. You can use this to compare two versions of ImageJ;
if they produce different measurements with the same input, feel free to
file a bug report about it using Help > Report a Bug. For details, see:
http://imagej.net/Bug_reporting_best_practicesRegards,
Curtis
On Wed, Oct 8, 2014 at 3:30 PM, ev34 <
[hidden email]> wrote:
> Hi,
>
> Two related questions:
>
> 1) A student is trying to open some fluorescence images he took using the
> latest version of imageJ (FIJI) and the screen shows up totally black. The
> goal is to quantify and compare fluorescence, so we need to figure out if
> this issue is related to the fact that he is using a really old Mac and Mac
> OSX 10.6.8 or some other setting that can be changed in the software? The
> pictures are saved in tiff format and when he took them they were very dim,
> but it was still possible to see them on the computer screen attached to
> the
> microscope. I can also open them on my computer just fine (latest version
> of
> imageJ, Mac OSX 10.8.5).
>
> 2) It's been a while since I used imageJ. I installed the newest FIJI
> version just recently - when opening fluorescence images they look really
> bright and grainy, not at all like they looked on the original monitor when
> I took the image. Why is this and how can I view them the normal way? I
> also
> notice that when I measure fluorescence (Analyze->Measure), the mean values
> that I get are very different from those used in earlier imageJ versions. I
> imagine this is related to the extreme brightness issue?
>
> Thank you!
>
> ~EV
>
>
>
> --
> View this message in context:
>
http://imagej.1557.x6.nabble.com/Opening-images-with-an-old-OSX-version-tp5009969.html> Sent from the ImageJ mailing list archive at Nabble.com.
>
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