Out of memory question

[Zachary Freyberg]

Dear all,
I have been working with large data sets consisting of approximately  
2000-2500 images acquired at 512x512 resolution. When using the latest  
release of Fiji, on attempting to open the respective image series  
consisting of one of these large data sets, I keep getting an out of  
memory error. I've tried resetting the memory to 1600 but even still,  
the program crashes and only opens around 400 out of the 2500 images.  
Are there any recommendations? Thank you in advance.

Best regards,
Zach Freyberg

[Yaseen Ladak]

Try opening  in the following manner : File > Import > Tiff as virtual stack

On 18 April 2012 22:08, Zachary Freyberg <[hidden email]> wrote:

> Dear all,
> I have been working with large data sets consisting of approximately
> 2000-2500 images acquired at 512x512 resolution. When using the latest
> release of Fiji, on attempting to open the respective image series
> consisting of one of these large data sets, I keep getting an out of memory
> error. I've tried resetting the memory to 1600 but even still, the program
> crashes and only opens around 400 out of the 2500 images. Are there any
> recommendations? Thank you in advance.
>
> Best regards,
> Zach Freyberg
>

[Zachary Freyberg]

Dear all,
Thank you for the advice regarding using a virtual stack after  
selecting import image sequence. While this has allowed me to open all  
2500 images in the stack, when I try to register them with Stackreg,  
it again stops with the log saying that it is out of memory.  
Similarly, I cannot do a plot Z-axis profile to look at intensity  
measurements. This occurs despite my attempts at increasing the memory  
allocation by increasing the memory to 1600. Is there a way to get out  
of this memory limitation? Thank you again for your help.

Best,
Zach Freyberg