> On Jun 13, 2016, at 8:29 AM, Aleksandr Mironov <
[hidden email]> wrote:
>
> Dear All,
>
> Is it possible to use different or alternating non-destructive overlays on different slices of a stack? Or for each slice the overlay should be flattened? For example, I want to put an overlay of parallel lines so that for each slice of a stack line orientation will alternate between two directions.
You can do this. The following macro provides an example.
-wayne
// Create a 4 slice stack
newImage("Overlay", "8-bit black", 500, 500, 4);
// Use Image>Overlay>Overlay Options to
// specify the line color and width
run("Overlay Options...", "stroke=red width=5 set”);
// Add a line to the overlay in each of the 4 slices
makeLine(117, 98, 385, 379);
run("Add Selection...");
run("Next Slice [>]");
makeLine(368, 100, 117, 405);
run("Add Selection...");
run("Next Slice [>]");
makeLine(93, 82, 365, 367);
run("Add Selection...");
run("Next Slice [>]");
makeLine(365, 77, 133, 372);
run("Add Selection...");
run("Select None”);
// Use Image>Overlay>List Elements to summarize
// the overlay. Note how the stack position of each
// line is shown in the “Pos” (position) column.
run("List Elements”);
// Use Image>Overlay>Flatten to flatten the stack
run("Duplicate...", "title=Flattened duplicate");
run("Flatten", "stack");
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