Overlay plots from plot profile

[Gareth Howell]

Hi

Is it possible to overlay plots generated with the Plot Profile tool?
We are interested in placing red and green fluorescence intensities along a line using this tool. Additionally would we be able to colour each line with either red or green. It would be quicker than having to create overlay plots in excel.

Thanks in advance

Gareth

[Jan Eglinger-5]

Hi Gareth,


Gareth Howell wrote:
> Is it possible to overlay plots generated with the Plot Profile tool?

You can set "Edit>Options>Profile Plot Options..." to a fixed y-axis,
then run Plot Profile on each channel and overlay the plots.
The following macro does this on an example image:

// ---------- Start Macro --------------
// Three Color Overlay Plot of a Line
run("Fluorescent Cells (400K)");
run("Profile Plot Options...", "width=450 height=200 minimum=0
maximum=255 fixed interpolate draw");
makeLine(226, 166, 327, 464);
run("Plot Profile");
run("Invert");
rename("red");
selectWindow("FluorescentCells.tif");
setSlice(2);
run("Plot Profile");
run("Invert");
rename("green");
selectWindow("FluorescentCells.tif");
setSlice(3);
run("Plot Profile");
run("Invert");
rename("blue");
run("Merge Channels...", "red=red green=green blue=blue gray=*None*
create keep");
// ---------- End Macro --------------

Hth,
Jan