Hi Pablo,
here is a rough idea for enhancing the particles of interest - it
still needs refining.
run("32-bit");
run("Smooth");
run("Square Root"); //reduces dynamic range
resetMinAndMax();
run("Thresholded Blur", "radius=3 threshold=6 softness=0.50
strength=3");
run("High Pass", "radius=20"); //radius depends on particle size
Then threshold it; maybe use "Find Maxima" with 'Above lower
threshold' and output 'Segmented Particles' to split overlapping
particles.
You will still need to filter the results of 'Analyze particles' by
size, circularity, etc.
The "Thresholded Blur" and "High Pass" filters are from
http://imagejdocu.tudor.lu/doku.php?id=plugin:startRemove any line break caused by the mailer in the line with run
("Thresholded Blur" ...
Michael
________________________________________________________________
On 26 Jul 2010, at 16:07, PabloCalva wrote:
> Hello,
>
> I am having a tough time separating my target particles from the
> material
> they are embedded in. I am trying to isolate the neurons, which are
> the
> white, sometimes gray spheres from the white/gray matter, which is
> the large
> blob of grayish material. The problem is that some of the neurons
> are not so
> much white as they are gray and match the unwanted areas, so that
> thresholding doesn't isolate them. If anyone knows of a way to
> approach this
> it would be greatly appreciated.
>
>
http://imagej.588099.n2.nabble.com/file/n5338089/
> r0000_z0000_c0000_t0000_Image0002-8bit.jpg
Locked
