Particle analysis

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Particle analysis

Breukers, C (TNW)
Dear all,
 
We have encounted a serious problem that is related to the particle
analysis in ImageJ.
We have developed a macro that counts bright cells on a dark background
in a monochrome 16-bit image.
The macro itself consists of some basic functions like rolling ball
background subtraction, maximum entropy threshold and particle analysis.
 
By coincidence we have found a deviation in number of counted cells
using the same macro in combination with a different version of ImageJ.
We tried version 1.37J and version 1.38V. We were suprised that there is
a difference in cell counting due to an updated version of ImageJ.
Maybe more people have encountered this?
 
Kind regards,
Christian Breukers

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Ing. Christian Breukers
Biophysical Engineering Group
Faculty of Science and Technology
University of Twente
Building 28 Zuidhorst
Room ZH273
P.O. Box 217,
7500 AE Enschede
The Netherlands
P.: +31 (0) 53 489 3021
P.: +31 (0) 53 489 3875 (Lab)
F.: +31 (0) 53 489 1105
E.: [hidden email] <mailto:[hidden email]>  
I.: http://bpe.tnw.utwente.nl <http://bpe.tnw.utwente.nl/>  
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