Particles tracking and fluorescence quantification in a stack

[GuillaumeG]

Hi everyone and thank you for this great forum full of great tips.

I have to analyze a time laps of particles whose localization and fluorescence change over time. So I would like to extract the path and the fluorescence for each particle. However, I don't know how to combine a particle tracking with the analyze particle pluggin. I am completely beginner in computation with imageJ script (and all other softwares actually). In other words, how can I assign a label to each particle along a stack and get there fluorescence and coordinates (I use Fiji) ?

In advance, thank you for all your advices.

Guillaume

[Krs5]

Dear Guillaume,

Did you have a look at the different Tracking plugins within Fiji (see: http://fiji.sc/Category:Tracking) or did you do a search for "Track" on the ImageJ Plugin website? There are many plugins available to help you tracking your particles and collecting data from these tracks. You might have to test a few to see what works for your data sets.

Best wishes

Kees

-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of GuillaumeG
Sent: 10 August 2014 05:39
To: [hidden email]
Subject: Particles tracking and fluorescence quantification in a stack

Hi everyone and thank you for this great forum full of great tips.

I have to analyze a time laps of particles whose localization and fluorescence change over time. So I would like to extract the path and the fluorescence for each particle. However, I don't know how to combine a particle tracking with the analyze particle pluggin. I am completely beginner in computation with imageJ script (and all other softwares actually). In other words, how can I assign a label to each particle along a stack and get there fluorescence and coordinates (I use Fiji) ?

In advance, thank you for all your advices.

Guillaume

<http://imagej.1557.x6.nabble.com/file/n5009078/Particles_10um.jpg>



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