Per channel contrast adjustment?

[Ed Siefker]

This is probably a simple question, but my google-fu is failing.

I have a red, green, and blue image I want to turn into a single
RGB image.  These images are of varying levels of intensity,
and all I care about is how visible all three are on the final image.

I can contrast each of them easily enough, and combine them
with Stack to RGB.  But I find myself repeating this procedure
over and over, trying to get the best image.  Is there a way I
can adjust the contrast of a single channel and preview it live?

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[Thomas Pengo]

You can convert the original RGB to a Composite

Image/Color/Make Composite

then you can adjust each channel independently (remember to Apply when
satisfied, for each channel) and then go back to RGB with

Image/Type/RGB Color

Cheers
Thomas





On 13 April 2016 at 15:24, Ed Siefker <[hidden email]> wrote:

> This is probably a simple question, but my google-fu is failing.
>
> I have a red, green, and blue image I want to turn into a single
> RGB image.  These images are of varying levels of intensity,
> and all I care about is how visible all three are on the final image.
>
> I can contrast each of them easily enough, and combine them
> with Stack to RGB.  But I find myself repeating this procedure
> over and over, trying to get the best image.  Is there a way I
> can adjust the contrast of a single channel and preview it live?
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

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[Jacqueline Ross]

Hi Ed,

You can merge the channels first and then adjust the levels of each channel independently by going to Image - Adjust - Colour Balance. Select the channel that you want to adjust from the drop-down box, e.g. Red) and the changes will only be implemented to that channel.

Kind regards,

Jacqui

Jacqueline Ross
Biomedical Imaging Microscopist
Biomedical Imaging Research Unit 
School of Medical Sciences 
Faculty of Medical & Health Sciences
The University of Auckland
Private Bag 92019
Auckland 1142, NEW ZEALAND

Tel: [phone]
Fax: [phone]

http://www.fmhs.auckland.ac.nz/sms/biru/


-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of Ed Siefker
Sent: Thursday, 14 April 2016 8:25 a.m.
To: [hidden email]
Subject: [FORGED] Per channel contrast adjustment?

This is probably a simple question, but my google-fu is failing.

I have a red, green, and blue image I want to turn into a single
RGB image.  These images are of varying levels of intensity,
and all I care about is how visible all three are on the final image.

I can contrast each of them easily enough, and combine them
with Stack to RGB.  But I find myself repeating this procedure
over and over, trying to get the best image.  Is there a way I
can adjust the contrast of a single channel and preview it live?

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html

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[Cammer, Michael]

I wrote this for someone last year.  Very likely there is a better way to do this (anyone want to tell me, I'd love to know!), but the code will give you an idea of how to do this to a whole directory.
http://microscopynotes.com/imagej/macros/composite-colors/user-selected-contrast-colors-names-save8bit.html
=========================================================================
 Michael Cammer, Microscopy Core & Skirball Institute, NYU Langone Medical Center
                      Cell:  914-309-3270     ** Office: Skirball 2nd Floor main office, back right **
          http://ocs.med.nyu.edu/microscopy & http://microscopynotes.com/

-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of Thomas Pengo
Sent: Wednesday, April 13, 2016 5:37 PM
To: [hidden email]
Subject: Re: Per channel contrast adjustment?

You can convert the original RGB to a Composite

Image/Color/Make Composite

then you can adjust each channel independently (remember to Apply when satisfied, for each channel) and then go back to RGB with

Image/Type/RGB Color

Cheers
Thomas





On 13 April 2016 at 15:24, Ed Siefker <[hidden email]> wrote:

> This is probably a simple question, but my google-fu is failing.
>
> I have a red, green, and blue image I want to turn into a single RGB
> image.  These images are of varying levels of intensity, and all I
> care about is how visible all three are on the final image.
>
> I can contrast each of them easily enough, and combine them with Stack
> to RGB.  But I find myself repeating this procedure over and over,
> trying to get the best image.  Is there a way I can adjust the
> contrast of a single channel and preview it live?
>
> --
> ImageJ mailing list:
> https://urldefense.proofpoint.com/v2/url?u=http-3A__imagej.nih.gov_ij_
> list.html&d=CwIBaQ&c=j5oPpO0eBH1iio48DtsedbOBGmuw5jHLjgvtN2r4ehE&r=oU_
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