Hi anonymous,
is Analyze>Set Scale what you want?
http://rsb.info.nih.gov/ij/docs/guide/146-30.html#toc-Subsection-30.8The scale applies to a whole stack, i.e., all images (slices) in the stack will have the same number of pixels per mm (or per µm, or whatever your sizes are).
Michael
________________________________________________________________
On May 13, 2014, at 13:38, bmm7 wrote:
> Dear All,
>
> I am trying to measure the lengths of objects along a path of a known
> distance. Each image is taken from a slightly different location (ie the
> pixels are different). There is a stack of images and I am forced to divide
> the "distance in pixels" by "known distance" for each image to normalise
> between the samples. It is very energy and time consuming. Is there an
> alternative method that i have overlooked? ImageJ should be able to do that
> automatically right?
>
> Thanks in advance,
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