Plot profile

[Mendoza Rodriguez]

Hello,
I would like to measure distances between regions of higher intensity  
by using the plot profile feature. To do that I draw a line over the  
image, I plot the profile and I use the multi-point tool to mark  
intensity peaks of interest. The problem is that if I use the  
"measure" command I get values based on the source image scaling, not  
on the "plot profile image". I can see that I get appropriate values  
by moving the cursor over the plot or by clicking on the list value,  
but I would like to retrieve only the values that I mark.
Any idea?
Mendo

[Tiago Ferreira-2]

Hi Mendo,

We've written a macro for a very similar purpose.
Have a look here:
<https://dl.dropbox.com/s/e9i5bihrxi9zc0x/Segment_Profile.ijm>

-tiago

On 2010.12.14, at 11:22 , Mendoza Rodriguez wrote:

> Hello,
> I would like to measure distances between regions of higher intensity  
> by using the plot profile feature. To do that I draw a line over the  
> image, I plot the profile and I use the multi-point tool to mark  
> intensity peaks of interest. The problem is that if I use the  
> "measure" command I get values based on the source image scaling, not  
> on the "plot profile image". I can see that I get appropriate values  
> by moving the cursor over the plot or by clicking on the list value,  
> but I would like to retrieve only the values that I mark.
> Any idea?
> Mendo

[Mendoza Rodriguez]

Hi Tiago,

Thank you for your help. I tried the plugin and unfortunately our images are too noisy to get dissent results. For this reason, I was planning on measuring the distance between peaks only (as opposed as measuring the width at a given threshold).

Regards
Mendo