Problem importing stack into TrakEM2

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Problem importing stack into TrakEM2

Rick Giuly
Hello All,

I'm attempting to load this stack into TrakEM2 (1.0a 2012-07-04)

http://cytoseg.googlecode.com/files/data.zip

First I open the stack in ImageJ, and it opens fine will no problem.

To import into TrakEM2, I right click in TrakEM2, select Import, Import
Stack, and select the stack that I just opened.

A window comes up that says:
Repair: Untitled 1
Image                                                  Nonexistent file path
out0000.png data 1 z=0.0 #8 /home/rgiuly/images/neuropil/data/
....

However the files do exist.

A few of the first slices do not open because of the Nonexistent file
path problem, and the rest of the slices load fine.

Appears to be a bug.

Any help is appreciated.

Thanks,

-Rick

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Re: Problem importing stack into TrakEM2

Albert Cardona-2
2012/10/19 Rick Giuly <[hidden email]>:

> Hello All,
>
> I'm attempting to load this stack into TrakEM2 (1.0a 2012-07-04)
>
> http://cytoseg.googlecode.com/files/data.zip
>
> First I open the stack in ImageJ, and it opens fine will no problem.
>
> To import into TrakEM2, I right click in TrakEM2, select Import, Import
> Stack, and select the stack that I just opened.
>
> A window comes up that says:
> Repair: Untitled 1
> Image                                                  Nonexistent file path
> out0000.png data 1 z=0.0 #8 /home/rgiuly/images/neuropil/data/
> ....
>
> However the files do exist.
>
> A few of the first slices do not open because of the Nonexistent file
> path problem, and the rest of the slices load fine.
>
> Appears to be a bug.
>
> Any help is appreciated.
>
> Thanks,
>
> -Rick



Rick,

while stacks are supported by TrakEM2, the best way to import a stack
is the following:

1. Save the stack as an image sequence with "File - Save As - Image Sequence".
2. Drag and drop the folder containing the image sequence onto the
fiji/ImageJ toolbar, and choose to import it as a virtual stack.
3. From the TrakEM2 canvas, right-click and select "Import - Import
Stack" and choose the virtual stack.

Now every slice of the stack will be imported as a separate image.
This is good for a number of reasons; namely TrakEM2 will be able to
work more efficiently.

Albert

--
http://albert.rierol.net
http://www.ini.uzh.ch/~acardona/

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Re: Problem importing stack into TrakEM2

Rick Giuly
> 2012/10/19 Rick Giuly<[hidden email]>:
>> Hello All,
>>
>> I'm attempting to load this stack into TrakEM2 (1.0a 2012-07-04)
>>
>> http://cytoseg.googlecode.com/files/data.zip
>>
>> First I open the stack in ImageJ, and it opens fine will no problem.
>>
>> To import into TrakEM2, I right click in TrakEM2, select Import, Import
>> Stack, and select the stack that I just opened.
>>
>> A window comes up that says:
>> Repair: Untitled 1
>> Image                                                  Nonexistent file path
>> out0000.png data 1 z=0.0 #8 /home/rgiuly/images/neuropil/data/
>> ....
>>
>> However the files do exist.
>>
>> A few of the first slices do not open because of the Nonexistent file
>> path problem, and the rest of the slices load fine.
>>
>> Appears to be a bug.
>>
>> Any help is appreciated.
>>
>> Thanks,
>>
>> -Rick
>
>
>
> Rick,
>
> while stacks are supported by TrakEM2, the best way to import a stack
> is the following:
>
> 1. Save the stack as an image sequence with "File - Save As - Image Sequence".
> 2. Drag and drop the folder containing the image sequence onto the
> fiji/ImageJ toolbar, and choose to import it as a virtual stack.
> 3. From the TrakEM2 canvas, right-click and select "Import - Import
> Stack" and choose the virtual stack.
>
> Now every slice of the stack will be imported as a separate image.
> This is good for a number of reasons; namely TrakEM2 will be able to
> work more efficiently.
>
> Albert
>



Hi Albert, using the Virtual Stack option to open a PNG stack wasn't
working for me. I was still getting similar errors.

However, I think I found a work around. If I save the stack as a single
AVI (using PNG compression), open that file, and then import to TrakEM2,
it works fine.

Best,
-Rick

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