Problem with Biovoxel's Extended Particle Analyzer

[Straub, Volko A. (Dr.)]

Hi,

I am trying to use the Extended Particle Analyzer to filter some objects in binary images. However, whatever I do, the Extended Particle Analyser doesn't appear to find any objects. To illustrate the problem, I have attached a small test image (test.tif). Using the standard Particle Analyzer using the macro code below on the test image (left image below) results in the expected image showing particles larger than 3 um in area (middle image). However, trying to run the Extended Particle Analyzer always results in a completely white image with no detected objects (right image).

Am I missing something obvious? Any suggestions/help would be very much appreciated.

Thanks,

Volko


Macro code applied to

run("Analyze Particles...", "size=3-Infinity show=Masks clear");
selectWindow("test");
run("Extended Particle Analyzer", "size=3-Infinity show=Masks redirect=None keep=None clear");

[cid:323e762e-f032-4ab9-b39e-47c99b4d6895]








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[Aryeh Weiss]

On 02/06/2017 10:27, Straub, Volko A. (Dr.) wrote:
> Hi,
>
> I am trying to use the Extended Particle Analyzer to filter some objects in binary images. However, whatever I do, the Extended Particle Analyser doesn't appear to find any objects. To illustrate the problem, I have attached a small test image (test.tif). Using the standard Particle Analyzer using the macro code below on the test image (left image below) results in the expected image showing particles larger than 3 um in area (middle image). However, trying to run the Extended Particle Analyzer always results in a completely white image with no detected objects (right image).
>
> Am I missing something obvious? Any suggestions/help would be very much appreciated.

The extended particle analyzer worked properly with your image on my  
machine (Fiji distribution, OSX 10.12.5)

You might try explicitly setting the various parameters in the filters,
because the extended particle analyzer  might remember a previous
setting that filters out the objects in your test image.

Best regards
--aryeh

> Thanks,
>
> Volko
>
>
> Macro code applied to
>
> run("Analyze Particles...", "size=3-Infinity show=Masks clear");
> selectWindow("test");
> run("Extended Particle Analyzer", "size=3-Infinity show=Masks redirect=None keep=None clear");
>
> [cid:323e762e-f032-4ab9-b39e-47c99b4d6895]
>
>
>
>
>
>
>
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

--
Aryeh Weiss
Faculty of Engineering
Bar Ilan University
Ramat Gan 52900 Israel

Ph:  972-3-5317638
FAX: [phone]

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