Problem with the Image Calculator

[María Victoria Pepe]

Hi!!

I´ve a problem with the Image Calculator. I wanted to evaluate the number
of bacteria in a z-stack and I decided to sum all the slices (Sum Slices)
and then make a mask of the bacteria, and use the AND function of the Image
Calculator to evaluate only the fluorescence that matches the mask. The
result of this operation gives me a dotted image. When I convert the image
to 16 bit, I don't have this problem, but I need to keep the image in 32
bit. I am not sure what mistake I am making. I wanted to know if you could
help me.

Thank you very much

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[Aryeh Weiss]

On 21/07/2020 02:02, María Victoria Pepe wrote:

> Hi!!
>
> I´ve a problem with the Image Calculator. I wanted to evaluate the number
> of bacteria in a z-stack and I decided to sum all the slices (Sum Slices)
> and then make a mask of the bacteria, and use the AND function of the Image
> Calculator to evaluate only the fluorescence that matches the mask. The
> result of this operation gives me a dotted image. When I convert the image
> to 16 bit, I don't have this problem, but I need to keep the image in 32
> bit. I am not sure what mistake I am making. I wanted to know if you could
> help me.
>
> Thank you very much
>

I suspect that you are using a logical bit by bit AND on a 32 bit image.
This will not work.

What I do in such cases is convert the mask to an image that contains
only 1 and 0 (eg by integer division by 255), and then
multiplying the two images, instead of logical AND.

--aryeh

--
Aryeh Weiss
Faculty of Engineering
Bar Ilan University
Ramat Gan 52900 Israel

Ph:  972-3-5317638
FAX: [phone]

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ImageJ mailing list: http://imagej.nih.gov/ij/list.html

[María Victoria Pepe]

Hi!!

This is a beautiful group!
Whenever I had a problem, you knew how to guide me!

Thank you so much for all the help!

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El lun., 20 jul. 2020 a las 23:48, Aryeh Weiss (<[hidden email]>)
escribió:

> On 21/07/2020 02:02, María Victoria Pepe wrote:
> > Hi!!
> >
> > I´ve a problem with the Image Calculator. I wanted to evaluate the number
> > of bacteria in a z-stack and I decided to sum all the slices (Sum Slices)
> > and then make a mask of the bacteria, and use the AND function of the
> Image
> > Calculator to evaluate only the fluorescence that matches the mask. The
> > result of this operation gives me a dotted image. When I convert the
> image
> > to 16 bit, I don't have this problem, but I need to keep the image in 32
> > bit. I am not sure what mistake I am making. I wanted to know if you
> could
> > help me.
> >
> > Thank you very much
> >
> I suspect that you are using a logical bit by bit AND on a 32 bit image.
> This will not work.
>
> What I do in such cases is convert the mask to an image that contains
> only 1 and 0 (eg by integer division by 255), and then
> multiplying the two images, instead of logical AND.
>
> --aryeh
>
> --
> Aryeh Weiss
> Faculty of Engineering
> Bar Ilan University
> Ramat Gan 52900 Israel
>
> Ph:  972-3-5317638
> FAX: [phone]
>
>

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ImageJ mailing list: http://imagej.nih.gov/ij/list.html