Profile from a XZ image

[Nicolas, Y. (Yves)]

Hello,
Has anybody tried to get a profile from a XZ confocal RGB image?
In my case, the threshold function can not really be used here to set the border between the surface and the outer surface (at least at this early stage) because the image has a black area on the top (from out of the sample) and on the bottom (deep in the sample).

I though of implementing the RGB profile macro by scanning the whole picture (line by line) and from each line profile, extracting the pixel position (x,z) where starts the sample surface border (here by selecting a pixel intensity value). A new image will contain these pixels from the border representing the profile of this xz image.
However I wonder if there is an easier way? Any ideas?
Thanks,
Yves

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