Hi,
I’m in George Christ’s lab at Wake Forest Institute for Regenerative Medicine. Our emphasis is in skeletal muscle tissue engineering. We would like to use imageJ to count the number of elongated myotubes in a 40 or 60X magnification. Additionally, we would also like to automate the cell counting of nuclei within each myotube (a myotube is formed when myoblasts fuse together creating elongated structures of 2 or more multi nuclei).
In a 60X FOV (I can share a representative image upon request), we would like to use imagej for counting the number of myotubes, quantifying the number of multi nuclei in each myotube and counting the total number of nuclei. Are there any plugins that any of you would recommend for this application?
Look forward to the feedback.
Thank you,
Mevan Siriwardane
Mevan Siriwardane, Ph.D.
Post-doctoral Fellow
Wake Forest School of Medicine
Institute for Regenerative Medicine
391 Technology Way
Winston-Salem, NC 27157
Ph: 201-898-8585
[hidden email]
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