Quantification of RAW images (Nikon NEF)

[Guenter Giese]

Dear list members,

 

I would like to quantify brightness of structures from images taken in RAW
format (Nikon NEF, no compression). I am not sure about data conversion /
processing regarding quantification / linearity issues.

 

Is there any ImageJ plugin (or other standard software) to convert the RAW
image to standard TIFF and /or to extract individual color channels from the
raw file directly without sacrificing linearity?

 

Is this issue covered in detail somewhere on the Web?

 

Regards,

 

Guenter

 

Dr. Guenter Giese
Light Microscopy Facility Manager
Dept. of Biomedical Optics
MPI fuer Medizinische Forschung
D-69120 Heidelberg, Germany
phone  xx49 or 0 -6221-486-360  fax -325
e-mail: [hidden email]

 

[Jarek Sacha-2]

On Tue, Jan 31, 2012 at 11:41 AM, Guenter Giese <
[hidden email]> wrote:

> Is there any ImageJ plugin (or other standard software) to convert the RAW
> image to standard TIFF and /or to extract individual color channels from
> the
> raw file directly without sacrificing linearity?
>
> Try ij-dcraw (http://ij-plugins.sourceforge.net/plugins/dcraw/index.html)
it gives several options how a RAW image can be converted.