Quantify fluorescence via ImageJ

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Quantify fluorescence via ImageJ

Jan Moch
Hello, I am new to ImageJ.
Our group researches C. elegans, my special interest are the fat depots. I want to quantify the amount of fat stored in the intestinum of the worm by staining the fat with a fluorescent dye. The resulting fluorescence is photographed under a epifluorescence microscope, producing 2D-bitmaps. I need to quantify this fluorescence. It is not sufficent to measure the mean, max and min intensity.

I would appreciate any help. Thanks, Jan