Quantifying Red and blue

[Ali123]

I am using a stain that stains blood vessel where muscel is red and stains collagen blue. I am looking for a plugin that will allow me to quantify how much red there is vs blue or how much blue there is vs red. Thanks for the help

[Joel Sheffield]

Hi,

If your colors are indeed pure red and blue, you can separate the individual
color planes by using Image>Color>Split Channels.  Then you can analyze each
separately.  My guess is that you will have a fair amount of bleed-through,
which you will have to compensate for.

However, take a look at Gabriel Landini's Color Deconvolution Plugin.  The
intro page shows you how to set up your own matrix for analysis if the ones
that he provides don't work.

http://www.dentistry.bham.ac.uk/landinig/software/cdeconv/cdeconv.html

Best of Luck

Joel


On Thu, Sep 3, 2009 at 7:03 PM, Ali123 <[hidden email]> wrote:

> I am using a stain that stains blood vessel where muscel is red and stains
> collagen blue. I am looking for a plugin that will allow me to quantify how
> much red there is vs blue or how much blue there is vs red. Thanks for the
> help
> --
> View this message in context:
> http://n2.nabble.com/Quantifying-Red-and-blue-tp3576682p3576682.html
> Sent from the ImageJ mailing list archive at Nabble.com.
>



--


Joel B. Sheffield, Ph.D
Department of Biology
Temple University
Philadelphia, PA 19122
Voice: 215 204 8839
e-mail: [hidden email]
URL:  http://astro.temple.edu/~jbs