Question on finding ROI

[gbarash]

Hello all,
I am a beginning user of imageJ API (and beginner image processor) and I
have a question about something that I am sure should be straightforward to
do:
I have the attached image and I need to find the x,y position of the
fiduciary mark in the top-left corner of the bead matrix in the image.
Ultimately I need to find the x,y positions of all beads in the matrix but
I assume once I find the corner mark at the top left I can calculate the
rest.
Is there a straightforward way to use imageJ API in a java utility to
ascertain the position of the fiducial mark?
Thanks!
Gilad

[Krs5]

Dear Gilad,

I am not sure what you try to do, but I assume you want to measure the intensity of your beads?

You might want to have a look at the DotBlotAnalyzer ToolSet. You can download it from http://imagej.nih.gov/ij/macros/toolsets/ and save it in the subdirectory macros/toolsets. More info can be found at http://image.bio.methods.free.fr/dotblot.html. By using the double arrow icon at the right of the ImageJ Tool Bar you can chose the toolset to load. You probably have to analyse the plate in two separate analyses as the macro assumes all columns to start at the same height.

Kees

Senior Experimental Officer
Centre for Core Biotechnology Services
College of Medicine, Biological Sciences and Psychology
http://www.le.ac.uk/biochem/microscopy/home.html




 
-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of Gilad Barash
Sent: 08 February 2012 03:54
To: [hidden email]
Subject: Question on finding ROI

Hello all,
I am a beginning user of imageJ API (and beginner image processor) and I have a question about something that I am sure should be straightforward to
do:
I have the attached image and I need to find the x,y position of the fiduciary mark in the top-left corner of the bead matrix in the image.
Ultimately I need to find the x,y positions of all beads in the matrix but I assume once I find the corner mark at the top left I can calculate the rest.
Is there a straightforward way to use imageJ API in a java utility to ascertain the position of the fiducial mark?
Thanks!
Gilad