Wolfgang,
I think it will be possible to convert a trace into numbers, once the trace
is a line which you can select cleanly. When thresholded and selected with
the wand tool, you may then save (Save-AS ...) the outline of the line as
X-Y coordinates. This will probably result in the outline of the trace. I.e.
The coordinates all around the line. So some editing will be in order. There
are some other ways to do this in ImageJ. (usually a lot of ways).
Then the data will be available as a text file or an excel file (edit menu/
options/ input-output and select the appropriate file extension.)
One program which can separate peaks and fit peaks etc is IGOR-Pro by
Wavemetrics. A free download of a trail is available from the Wavemetrics
web site. There is a bit of a learning curve, but it is fast capable, and
was originally written to handle data with peaks, which it does very well.
HTH
--
Noel Goldsmith
DSTO
506 Lorimer Street
Port Melbourne
Victoria
Australia
3207
Phone 613 96267527
Mobile 0428364003
Fax 613 96267718
Email
[hidden email]
On 4/2/08 4:00 PM, "IMAGEJ automatic digest system" <
[hidden email]>
wrote:
> There are 2 messages totalling 82 lines in this issue.
>
> Topics of the day:
>
> 1. Peak fitting with ImageJ (2)
>
> ----------------------------------------------------------------------
>
> Date: Sun, 3 Feb 2008 14:55:44 +0100
> From: Wolfgang Schechinger <
[hidden email]>
> Subject: Peak fitting with ImageJ
>
> Dear ImageJ experts,
>
> is there a way to do peak fitting within ImageJ? I habe obtained some
> chromatogram-like curves with the Crtl+1,2,3 sequence and would like to
> estimate if there is more than one signal hidden in one peak (i.e. if one peak
> may be expressed as the sum of two, partially conincidental, peaks).
> Alternatively, is there a way to convert and save these curves as numbers in
> e.g. a csv or text file that one may process with some third party software
> (any recommendation for which software might be useful are highly appreciated
> as well)
>
> Many thanks!
>
> Wolfgang
IMPORTANT: This email remains the property of the Australian Defence Organisation and is subject to the jurisdiction of section 70 of the CRIMES ACT 1914. If you have received this email in error, you are requested to contact the sender and delete the email.
Locked
