RGB Extension of Analyze Particle?

[Claude Bonnard]

Hello,

Does someone have an idea on how to do the following:

-From an RGB image, select one color and define from it a mask by thresholding
or other mode of selection. This "Mask" image would contain binary objects that
could be analyzed as described in ImageJ decumentation.
-Instead of determining the values for the selected color (Area, Mean etc) for
each particle defined this way, what I would like is to be able to determin,
for each particle the values in the R, G and B channels. The mask would be here
to create those multiple ROI applying to every color (not a Gray-valoe
replacing the colors).

If you have an idea on how to do this, or if you want to share your method, I
would be very glad.

Many thanks,
Claude


--
Claude Bonnard Ph.D.
ISREC (Swiss Institute for Experimental Cancer Research)
Microscopy, Imaging and Morphology Core Facility [MIM]
Lab F216
Ch des Boveresses 155
CH-1066 Epalinges
Switzerland
email: [hidden email]
phone: [41-21]-692-5891
  fax: [41-21]-652-6933

[Gabriel Landini]

On Tuesday 20 February 2007 13:04:22 Claude Bonnard wrote:
> -From an RGB image, select one color and define from it a mask by
> thresholding or other mode of selection. This "Mask" image would contain
> binary objects that could be analyzed as described in ImageJ decumentation.
> -Instead of determining the values for the selected color (Area, Mean etc)
> for each particle defined this way, what I would like is to be able to
> determin, for each particle the values in the R, G and B channels. The mask
> would be here to create those multiple ROI applying to every color (not a
> Gray-valoe replacing the colors).

Use some method (colour thresholding?) to get your binary image holding the
particles.
Split the rgb image into 3 planes (R, G & B)
Use the binary image with the Particle Analyzer (or Particles8_Plus) and
use "redirect" to the R image.
Repeat the last step for the G and B images.

Now you should have the greyscale statistics for each image plane which you
could merge using some spreadsheet program.

G.