ROI color coder

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ROI color coder

inam1
Hi 
I need help with using ROI color coder. 
What i am doing is 
-thresholding image
-add selection to create NP boarder
-adding into ROI manager 
-flatting
-and using ROI color coder for selected and fllatened image . 
But it is not working. can you please tel me where m making a mistake. 


Many Thanks