Re: Analysing x-z and y-z slices

[Glen MacDonald]

Dear Graham,
Set the lateral and axial scales under Image/Properties
then reslice the image in your preferred axis:
Draw a rectangular ROI on a representative xy plane,
Image/Stacks/Reslice...
select the direction to reslice, e.g. "top", "Left", etc.
result is a new stack from the x,z or y,z orthogonal plane.

Glen
On Jan 29, 2008, at 9:00 PM, IMAGEJ automatic digest system wrote:

>
> From: Graham Wright <[hidden email]>
> Date: January 28, 2008 7:17:15 PM PST (CA)
> Subject: Analysing x-z and y-z slices
>
>
> Hello,
> I have a z-stack acquired on a Zeiss LSM 510 META confocal of a  
> sample in 2 channels (single time point). I would like to be able  
> to extract x-z and y-z slices so that I can analyse the  
> fluorescence intensity across them. Is this possible and/or does  
> anyone know how to do this in ImageJ?
> Thanks in advance for your help,
> Graham
>
> -----
> Graham Wright
> Microscopy & Imaging Facility
>
> Temasek Life Sciences Laboratory
> 1 Research Link
> National University of Singapore
> Singapore 117604
>
> P:    [phone]
> M:   [phone]
> E:     [hidden email]