ImageJ Interest Group <[log in to unmask]>
Sender: ImageJ Interest Group <[log in to unmask]>
From: Martyn Quinn <[log in to unmask]>
Subject: 'Analyze Particles' Problem
Hello,
I'm attempting to use the Analyze Particles feature to get a list of X/Y
coords for use in Ripley's K analysis.
I have an 800x800 pixel image (8 bit) with two clusters of 6nm gold.
Threshold is adjusted
Analyze 'Set Measurements' is ticked for 'Center of Mass' to 8 decimal
places
Analyze Particles (size 3-infinity, display results ticked)
Comes up with "Analyze Particles....0.406 secs, 1.6 million pixels/second"
But does not display any results whatsoever
Any help greatly appreciated on this.
Martyn
Just as guess, but is the image calibrated to cm? Your particles would be smaller than that, and yield no results. Try to set the scale to pixels or even nm via the Image\properties or Analyze\set scale routines.
Also, Analyze Particles requires thresholding or a binary image.
__
Vytas Bindokas, Ph.D.
Research Assoc. / Assoc. Prof.,
Director, BSD Light Microscopy Core Facility
Dept Neurobiol Pharmacol Physiol MC0926
947 E 58th Street
The University of Chicago
Chicago IL 60637
Room 1007 (CLSC)
773-702-4875
email
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web site for LMCF:
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