Re: Calculation of Image J parameters...VERY URGENT, PLEASE AND THANK YOU!

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Re: Calculation of Image J parameters...VERY URGENT, PLEASE AND THANK YOU!

ctrueden
Hi Geology Guy,

Your links are broken (Nabble returns a 404 for each), so I could not look
at them to figure out what exactly you are asking. So here is my general
advice: store your ROIs ("particles") using the ROI Manager. You can make
Analyze Particles do this by checking the "Add to Manager" box. Once your
ROIs are in the ROI Manager, you can save them using More > Save..., and
measure them by pressing Deselect and then Measure. If you then remove some
ROIs (deleting the undesirable ones as you said), you can then measure the
remaining ones using the Measure button again.

You can use the Plugins > Macros > Record command to record a macro of
operations that you perform in ImageJ. This allows you to build up a
repeatable analysis, redoing the same steps again later. This is invaluable
if you perform a complex series of steps, which you later realize needs
tweaking -- you can edit the macro with the tweaked steps and rerun it.

> My main problem now is that I deleted some of the particles as
> requested by my Professor from the list after the data was provided by
> Image J.

If you need to delete some ROIs according to data from the Results table
measurements, it is probably easiest to write a macro. Each row of the
Results table corresponds to the same index in the ROI Manager (at least
initially). However, if you have not written macros before, getting it
working before the 31st may be a tall order. I suggest seeking assistance
from a programmer in your group, if you have one.

HTH,
Curtis



On Fri, Jul 26, 2013 at 8:42 PM, Geology Guy <[hidden email]> wrote:

> Good Evening Everyone,
> I am trying to measure the parameters of 100 particles in Image J and under
> "Analyze Particles", I also selected the "Summary option". My main problem
> now is that I deleted some of the particles as requested by my Professor
> from the list after the data was provided by Image J. This makes the
> "Summary" data not useful anymore as they do not reflect all the 100
> particles I started with originally. I measured all the parameters under
> "Set measurement" for each particle so now I need to produce a new summary
> data as the values would be used in plotting graphs. I have figured out how
> to Area which is just a simple addition of the areas of each particle.
> Other
> parameters that are in the summary that I cannot determine from the
> particles data are the following:
>
> Angle W06.08_20_ParticleData.xls
> <http://imagej.1557.x6.nabble.com/file/n5004169/W06.08_20_ParticleData.xls
> >
> W06.08_20_Summary_of_Particle_Data.xls
> <
> http://imagej.1557.x6.nabble.com/file/n5004169/W06.08_20_Summary_of_Particle_Data.xls
> >
>
>
> Perim.
> Major
> Minor
> Circ.
> Solidity
> Feret
> FeretX
> FeretY
> FeretAngle
> MinFeret
> IntDen
> Median
> Skew
> Kurt
>
>
> I have attached 2 Excel spreadsheets. The first shows my original particles
> data and the highlighted rows in yellow are rows that I have deleted their
> data as they are bad data. I am working on my research that is due on the
> 31st of this month so this is very urgent. Thank you very much everyone for
> your assistance.
>
>
>
>
> --
> View this message in context:
> http://imagej.1557.x6.nabble.com/Calculation-of-Image-J-parameters-VERY-URGENT-PLEASE-AND-THANK-YOU-tp5004169.html
> Sent from the ImageJ mailing list archive at Nabble.com.
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

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Re: Calculation of Image J parameters...VERY URGENT, PLEASE AND THANK YOU!

dscho
Hi Guy,

On Fri, 26 Jul 2013, Geology Guy wrote:

> I am trying to measure the parameters of 100 particles in Image J and
> under "Analyze Particles", I also selected the "Summary option". My main
> problem now is that I deleted some of the particles as requested by my
> Professor from the list after the data was provided by Image J. This
> makes the "Summary" data not useful anymore as they do not reflect all
> the 100 particles I started with originally.

This sounds dangerously like removing particles "because they don't look
good". I strongly suggest finding a way to filter the particles by
appropriate ways (see e.g.
http://www.uab.edu/researchintegrityandimages/guidelines/list.html). That
way, the particle analyzer will just take those particles into account
that you want to analyze, your image analysis will be proper, and the
summary *will* be correct.

If you cannot come up with a way to filter the images globally (i.e.
non-biased) you are entering very dangerous terrain, ethically.

Ciao,
Johannes

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