Re: [EXTERNAL] Re: rendering a 3D image from side views only
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Re: [EXTERNAL] Re: rendering a 3D image from side views only
 
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Hi Bill
For imaging plant roots in situ have you considered neutron radiography/tomography? There has already been some work done along those lines and given the Constrasts generated by H2O and or D2O there is a lot you can see that is hard to image with X-rays (although the resolution is poorer). As with the synchrotrons all This requires is a beam-time proposal to one of several neutron facilities. Btw., with respect to photogrammetry, the specific technique used is often called "structure from motion" or SFM. There is freeware available for this as well as the Agisoft And other commercial products. -- Larry Lawrence M. Anovitz Senior Research Scientist Geochemistry and Interfacial Sciences Group Chemical Sciences Division Oak Ridge National Laboratory MS 6110, PO Box 2008 1 Bethel Valley Rd. Oak Ridge, TN, 37831-6110 865-574-5034 On 10/24/19, 12:55 PM, "Stein Rørvik" <[hidden email]> wrote: A comment to my own post; I looked on the Agisoft website and they are explaining how to overcome the problem with using a fixed camera and rotating object in a tutorial explaining some background masking functionality, which was introduced recently. However, this might be difficult to do with such a highly detailed object a plant root is. You might therefore be better off with a tomography based solution, depending on your budget. One tomography scan might cost the same as one SLR camera, or twice the cost of a license for the photogrammetry software. A great advantage with tomography data though is that it can be processed directly in ImageJ, while the photogrammetry software will produce a point cloud or surface model which needs to be converted. Stein -----Original Message----- From: ImageJ Interest Group <[hidden email]> On Behalf Of Stein Rørvik Sent: 24. oktober 2019 16:10 To: [hidden email] Subject: Re: rendering a 3D image from side views only Michael is right that in what you need is photogrammetry, not tomography. The tomography requires that your images are projections; that is, an image that represents some kind of sum of density values along a path, like an X-Ray image. That will not be the case for a "normal" photographic image. I have done a lot of X-ray microtomography but also some photogrammetry of various objects. For photogrammetry, I have used a commercial software called Agisoft PhotoScan that is easy to use and give excellent results. You need something like 500 images to get a reasonably good dataset, probably more in your case. So you need a rig with automated rotation and image acquisition for reproducible results. It is also probably possible to find free / open source software for doing this, but I have not checked that out since the Agisoft software works so well. If you only have a few samples and do not want to invest your time in setting up a rig, you can take thousands of images in less than an hour with a normal SLR and a big memory card. Just walk around your object and snap random photos at as many angles as you have the patience to do. Then feed it into the software and the rest is automatic. A disadvantage with Agisoft PhotoScan is that it requires your scene to be fixed and the camera moving. So it will NOT work well with a setup using a fixed camera and rotating object. The reason is that it uses details in the surroundings of the object to calculate the orientation of the camera towards the object. That might have changed in recent updates though; I have not checked recently. Stein -----Original Message----- From: ImageJ Interest Group <[hidden email]> On Behalf Of Dr. Michael DOUBE Sent: 23. oktober 2019 02:50 To: [hidden email] Subject: Re: rendering a 3D image from side views only Hi Bill, What you want is photogrammetry, which is the process of generating surface models (2.5D if you like) from a series of photographs. It's a popular technique in museum digital curation because it's no destructive and the hardware requirements are minimal. In my current department, a whole Omura's whale skeleton was digitised with just a digital SLR camera and some software (I don't know which one, sorry). You are likely to need many more photographs than you think, because you have to gather photons from every surface, otherwise surface regions will be missing from the final mesh. There are a few libraries out there that might be helpful for you - not sure if any are implemented in ImageJ. As others have pointed out, tomography requires images projected through the complete thickness of the sample for reconstruction at many angles - usually 1 - 3 thousand per full rotation, but you can get away with fewer with special reconstruction algorithms. There is a group in the UK, at the Hounsfield lab, doing X-ray microtomogrpahy of root systems. Would be worth checking them out. Hope it helps, Michael On 22/10/2019 04:38, BananaBoy wrote: Hi, I'm pretty new to imageJ and image analysis in general but I have a question that I cannot seem to find a clear answer to which is the following: I would like to construct a 3D model of a plant's root system by taking side views (and potentially a top view) images. For obvious reasons, I cannot create a stack of slices along the vertical axis. Is this possible? For example, I could create a setup where the plant is at a fixed position from the camera (with naked and clean roots hanging in the air). I could then rotate the plant 360 degrees in increments of 10 degrees, so 35 pictures in total. Is ImageJ capable of rendering a 3D model of this root system? 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