Re: FWHM measurement of puncta

[dpoburko]

Hi All,

  I would like to measure the FWHM diameter of a number of fluorescent
puncta. I was looking for a pre-existing pluggin or macro. I came across
Adrian's FWHM, but it doesn't appear in my plugins list when I install
it. Does anyone know of any other macros or pluggins for FWHM measurements?

Tx,
Damon

--

Damon Poburko, PhD
Postdoctoral Research Fellow
Stanford University School of Medicine
Dept. of Molecular & Cellular Physiology
279 Campus Dr., Beckman B103, Stanford, CA 94305
Ph: 650 725 7564, fax: [phone]

[Wayne Rasband]

On Aug 4, 2009, at 4:25 PM, Damon Poburko wrote:

> Hi All,
>
>  I would like to measure the FWHM diameter of a number of fluorescent
> puncta. I was looking for a pre-existing pluggin or macro. I came
> across Adrian's FWHM, but it doesn't appear in my plugins list when I
> install it. Does anyone know of any other macros or pluggins for FWHM
> measurements?

The documentation for Adrian's FWHM at
<http://rsbweb.nih.gov/ij/plugins/fwhm/> says it is installed in the
Analyze>Tools menu, not in the Plugins menu. With ImageJ 1.43, you can
install this plugin by dragging "Adrian_FWHM.jar" from a browser window
and dropping it on the ImageJ window.

-wayne