Re: IMAGEJ Digest - 22 Jul 2009 to 23 Jul 2009 (#2009-201)

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Re: IMAGEJ Digest - 22 Jul 2009 to 23 Jul 2009 (#2009-201)

Daniel James White
On 24 Jul 2009, at 06:00, IMAGEJ automatic digest system <[hidden email]
 > wrote:

> Date:    Thu, 23 Jul 2009 13:04:33 -0700
> From:    Giulia Falivelli <[hidden email]>
> Subject: Colocalization thresholds
>
> Hi all,
>
>  I just started using Image J and I am trying to do a colocalization
>  analysis using the plugin "Colocalization thresholds". I used the z-
> stack
> of
>  my images (2 channels), but I don't fully understand all the output  
> values
>  that I have in the file Results.
>  Can somebody help me to understand the meaning (or pointing me to the
> right
>  source) of: Rtotal, Rcoloc, R<threshold, M1, M2, tM1, tM2, Ncoloc.
>
>  The tutorial that I found here
>  http://pacific.mpi-cbg.de/wiki/index.php/ColocalizationAnalysis
>   was not too helpful in understanding these values
>

Read the original
Paper by coates et al and references therein. It is linked to on that  
fiji wiki page!

http://pacific.mpi-cbg.de/wiki/index.php/Image:Costes_etalColoc.pdf

Cheers

Dan
>
>  Thanks
>
>  Giulia