Re: Mean density question

[Dearolf, Jennifer]

Greetings!

I am new to this listserv, but I have been a long time NIH Image user and current Scion Image user.  I would like to switch over to Image J, but I am having a problem.  I use the mean density measurement in my research, and my problem is that in Image J, black is 0 and white is 256.  In Scion, black is 256 and white is 0.  Does anyone know how to invert the scale in Image J such that black is 256 and white is 0?  I know that I could just set up a spreadsheet in Excel to subtract the density measurement provided by Image J from 256, but it would be nice if the program would make the measurement using a scale that is equivalent to Scion.

Thanks so much for your help!

Sincerely,
Jenn

Jennifer Dearolf
Associate Professor
Hendrix College
1600 Washington Avenue
Conway, AR 72032
[phone] office
[phone] fax


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[Rasband, Wayne (NIH/NIMH) [E]]

On Jul 1, 2010, at 11:07 AM, Dearolf, Jennifer wrote:

> Greetings!
>
> I am new to this listserv, but I have been a long time NIH Image user and current Scion Image user.  I would like to switch over to Image J, but I am having a problem.  I use the mean density measurement in my research, and my problem is that in Image J, black is 0 and white is 256.  In Scion, black is 256 and white is 0.  Does anyone know how to invert the scale in Image J such that black is 256 and white is 0?  I know that I could just set up a spreadsheet in Excel to subtract the density measurement provided by Image J from 256, but it would be nice if the program would make the measurement using a scale that is equivalent to Scion.
>
> Thanks so much for your help!

Check "Use inverting lookup table" in the Edit>Options>Appearance dialog box. Note that this only works with 8-bit images.

-wayne