Hi Michael
I tried to attached a file, but it is too large even when compressed. Interestingly, when looking for a suitably sized file.... I noticed that the issue was not apparent with my smaller sized files. The scale bar was correct.
Is there any way I could upload the problem images to you or image J list?
Thank you
Sindy Kueh, PhD| Research Officer
Anatomy and Cell Biology|School of Medicine
T: 02-4620 3938| F: 02 4620 3890| E:
[hidden email]
Building 30, Goldsmith Avenue|Campbelltown NSW 2560
Mailing address: Locked Bag 1797,Penrith NSW 2751
westernsydney.edu.au
-----Original Message-----
From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of Cammer, Michael
Sent: Friday, 21 October 2016 2:15 PM
To:
[hidden email]
Subject: Re: Scale Bar LSM format image
In our experience with ImageJ, LOCI plugins or Bioformats version, and Zen 2009, as long as the lens was set correctly in the Zen software, the spatial scales and time intervals import fine.
Perhaps you could post one of the native lsm files?
_________________________________________
Michael Cammer, Optical Microscopy Specialist
http://ocs.med.nyu.edu/microscopy http://microscopynotes.com/Cell: (914) 309-3270
________________________________________
From: ImageJ Interest Group [
[hidden email]] on behalf of Sindy Kueh [
[hidden email]]
Sent: Thursday, October 20, 2016 9:11 PM
To:
[hidden email]
Subject: Re: Scale Bar LSM format image
Dear Image J users
I would like to add scale bar to images captured using the ZEN 2009 software. Images are store as LSM files. Unfortunately, the output scale seemed to be incorrect.
I have attached a picture of muscle fibres, showing the scale bar on bottom left which is incorrect: each fibre diameter is estimated to be in the range of 20-40uM, Do I need to calibrate Fiji - pixel to uM?
Thank you
Sindy
Sindy Kueh, PhD| Research Officer
Anatomy and Cell Biology|School of Medicine
T: 02-4620 3938| F: 02 4620 3890| E:
[hidden email]
Building 30, Goldsmith Avenue|Campbelltown NSW 2560
Mailing address: Locked Bag 1797,Penrith NSW 2751
westernsydney.edu.au
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