Dear all,
I would like to have your help in this case:
I am measuring size of particles in images by use of this macro:
run("8-bit");
run("Rename...", "title=Cievy.bmp");
run("Duplicate...", "title=Cievy.bmp-copy");
run("Gaussian Blur...", "sigma=2");
run("Multiply...", "value=2.550");
run("Add...", "value=125");
run("Maximum Entropy Threshold");
run("Set Measurements...", "area perimeter feret's redirect=None decimal=3");
run("Analyze Particles...", "size=1-Infinity circularity=0.00-1.00 show=Outlines display");
When Set scale... is in default value macro works but all measurements are done in pixel size. When I specified Distance in pixels = 99, Known distance = 0.05 and Unit of length = mm macro failed to measure analyzed particles.
Any hint, please?
Regards
Vladimir
Assoc.Prof.Vladimir Hegyi,MD,PhD.
Dept. of Pediatric Dermatovenerology
School of Medicine
Comenius University
Limbova 1
833 40 Bratislava
Slovak Republic
e-mail:
[hidden email]
[hidden email]
www.dermatology.sk
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