Re: cell counting help needed

[Aryeh Weiss]

On 19/11/2015 11:29 PM, Mayur Kajla wrote:
> Seeking help with counting of GFP+ cells in the attached image. This is pseudo colored DAPI (nuclei only) and some cells showing GFP dots.
> How can both cell types be counted using image J. Thanks
>
> Mayur
>
> University of Wisconsin-Madison
>

Here is something quick to get you started:

selectWindow("c636 merge.png");
run("Split Channels");
selectWindow("c636 merge.png (red)");
run("Gaussian Blur...", "sigma=4");
// from the GDSC plugins -- they have an update site
run("Difference of Gaussians", "  sigma1=10 sigma2=2 enhance");
run("Find Maxima...", "noise=5 output=[Point Selection]");
roiManager("Add");

The lines are a bit of a bother -- you can use the blue and green
channels to fix that.

I have to run, but when I a chance, I will clean it up a bit.
Perhaps you can post an image of what you want to detect in the green
channel, which is less obvious to me.

--aryeh

--
Aryeh Weiss
Faculty of Engineering
Bar Ilan University
Ramat Gan 52900 Israel

Ph:  972-3-5317638
FAX: [phone]

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