Hi Elena,
Thanks for your answer.
I still suspect that Costes' thresholding might not be the method used by Coloc2 to define thresholded Manders coefficients. Indeed Coloc 2 does not give the same threshold values as JaCoP, which according to (Bolte & Cordelières, 2006) uses Costes' method.
I will follow your advice and go to the forum.
A nice day to you all !
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