Re: phase from Array.fourier()

[dpoburkoSFU]

Hi all,

    I am trying to examine Ca2+ oscillations in time lapse images of
cultured cells, specifically looking at the degree to which oscillations
in neighbouring cells are synchronized. I have been able to use the
Array.fourier() function to automate the analysis of the oscillation
frequency (and ampltidue... I think).

     Is it possible to get the phase data from the Array.fourier()
function somehow, or could the function be modified to get this information?

Thanks,
Damon

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Assistant Professor | Biomedical Physiology & Kinesiology | Simon Fraser University
Member | Centre for Cell Biology, Development, and Disease | Simon Fraser University
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[Jacob Keller-2]

Jay Unruh from Stowers has a plugin that does just what you want--you can
get those even in the updating website choices in IJ. It's called something
like stack.temporal.FFT or similar, then follow that by complex.amp or
complex.phase to extract what you want from the complex stack. He has Excel
sheets describing all of the plugins. There is also a graphical description
of the plugin in the supplemental of this paper:

http://dx.doi.org/10.1016/j.molcel.2016.09.001

Jacob Keller

On Wed, Dec 7, 2016 at 6:08 PM, Damon Poburko <[hidden email]> wrote:

> Hi all,
>
>    I am trying to examine Ca2+ oscillations in time lapse images of
> cultured cells, specifically looking at the degree to which oscillations in
> neighbouring cells are synchronized. I have been able to use the
> Array.fourier() function to automate the analysis of the oscillation
> frequency (and ampltidue... I think).
>
>     Is it possible to get the phase data from the Array.fourier() function
> somehow, or could the function be modified to get this information?
>
> Thanks,
> Damon
>
> --
>
> Assistant Professor | Biomedical Physiology & Kinesiology | Simon Fraser
> University
> Member | Centre for Cell Biology, Development, and Disease | Simon Fraser
> University
> Tel: [phone] | Fax: [phone] | Office L8004
> Mail to: Room K9625, 8888 University Drive, Burnaby BC V5A 1S6
> http://www.sfu.ca/bpk/faculty_directory/poburko.html
>
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

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ImageJ mailing list: http://imagej.nih.gov/ij/list.html

[Herbie]

Good day Damon,

you may have a look at "nr realft"

<http://www.gluender.de/Miscellanea/MiscTexts/UtilitiesText.html>

described here:

<http://www.gluender.de/Miscellanea/MiscTexts/MiscExcerpts
/ReadMe_FFT.html>

Best

Herbie

::::::::::::::::::::::::::::::::::::::::::
Am 08.12.16 um 00:08 schrieb Damon Poburko:

> Hi all,
>
>    I am trying to examine Ca2+ oscillations in time lapse images of
> cultured cells, specifically looking at the degree to which oscillations
> in neighbouring cells are synchronized. I have been able to use the
> Array.fourier() function to automate the analysis of the oscillation
> frequency (and ampltidue... I think).
>
>     Is it possible to get the phase data from the Array.fourier()
> function somehow, or could the function be modified to get this
> information?
>
> Thanks,
> Damon
>

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